CYTO OF THE EXTREME
From ETC to ESS - Transforming our commitment to single-cell science!
Continue readingVisual metrics in BD Multi-Omics: Your key to finding the impossible!
The CytohubIt has always been difficult to find the impossible, but what makes it so? To unravel the secrets of single-cells it is important to start the experiment with viable and...
Continue readingComprehensive phenotyping of Human Dendritic Cells and Monocytes
Scientific PublicationIntroduction Dendritic cells (DC) and monocytes, along with macrophages, constitute the Mononuclear Phagocyte System (MPS), which scans for and provides the first line of defence against invading pathogens. Some cells...
Continue readingT cell cutoff values for identifying COVID-19 patients at...
The CytohubImportance of CD4 and CD8 T cells CD4 and CD8 T cells are important in viral killing and antibody response. T-cell subsets typically decrease with increasing disease severity in COVID-19 patients.1 Upon admission in hospitals, a major challenge...
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Which BD Fluorochrome family do you belong to ?
The CytohubWhich BD Fluorochrome family do you belong to ?
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BD™ AbSeq protocol using the nano-well cartridge-based BD Rhapsody™ platform
Scientific PublicationThe BD™ AbSeq oligonucleotide-conjugated antibodies The BD™ AbSeq oligonucleotide-conjugated antibodies, when incorporated into a single-cell mRNA-sequencing experiment, can yield combined transcript and protein expression data. Such data can provide vital insights into cellular processes and yield insightful information about gene expression. This article by Erickson, et.al provides a detailed experimental protocol for the combined analysis […]
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Confronting the EU in vitro diagnostic regulation (EU IVDR) – Lift the fog!
News & innovationsThe EU In Vitro Diagnostic Medical Device Regulation 2017/746 (IVDR) The EU In Vitro Diagnostic Medical Device Regulation 2017/746 (IVDR) took effect on 26 May 2017, and a transitional period of 5 years is in force till 26 May 2022. The IVDR replaces the current EU In Vitro Diagnostic Medical Device Directive (IVDD) (98/79/EC) to ensure a higher level […]
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Flow cytometry and 3D bioprinting of human tissue models – BD and CTIBiotech
News & innovations, The CytohubBD and CTIBiotech CTIBiotech is a young biotech company that specialises in human tissue bio-assay development. The company specialises in building 3D bio-printed human tissue models that are used in numerous applications in a variety of fields. Areas of application of these models include dermato-cosmetic research, cancer research, biomedical and pharmaceutical development. Flow cytometry and […]
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BD Multi-Omics: Now even the impossible can be found
News & innovations, The CytohubBreaking news! The impossible is no longer impossible! BigFoot, your friendly, furry, but hard-to-find friend is hiding. Finding him is like finding your next scientific breakthrough. It requires patience, long working hours and much effort. But finding the impossible need not be hard. BD Multi-Omics solutions are specially created to help you unravel complex biological […]
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CRISPR screens using targeted single-cell RNA sequencing
Scientific PublicationCell perturbation using the CRISPR technique Earlier on www.flowcytometry-news.com, we featured an article on the use of the BD Rhapsody™ system to perform highly scalable and more sensitive CRISPR screens. In cell perturbation using the CRISPR technique, single guide RNAs (sgRNAs) are used to guide the endonuclease Cas9 to create a targeted double-strand break, the […]
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OMIP-068: High-dimensional characterization of global and antigen-specific B cells in chronic infection
Scientific PublicationOMIP-068: High-dimensional characterization In this OMIP (Optimised Multicolour Immunophenotyping Panel), the authors describe a 24-color panel to characterise antigen-specific B cells and for the precise delineation of B cell subsets in chronic infection.1 B cells play an important role in infection response through the production of antibodies, antigen presentation and secretion of cytokines. The antibodies […]
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OMIP-070: NKp46-based 27-color phenotyping to define natural killer cells isolated from human tumor tissues
Scientific PublicationOptimised Multicolour Immunophenotyping Panel In this OMIP (Optimised Multicolour Immunophenotyping Panel), the authors describe a 27-color phenotyping panel to study NK, or Natural Killer cells.1 The interest in NK cells began in the 1970s when it was discovered that they possess the ability to destroy tumor cells, and could play a role in controlling malignancies […]
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Multicolour flow cytometry panels for COVID-19 research!
The CytohubEnabling your research into the immune response to COVID-19 There is now a pressing need to unravel the biological mechanisms underlying COVID-19 infections, as the pandemic continues to spread all around the world. Research studies have shown that there is an alteration in the composition of immune cells and dysfunctional immune response in COVID-19 patients. […]
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