Dendritic cells (DCs), a vital part of the innate and adaptive immune system, are antigen-sensing and presenting cells uniquely possessing the ability to induce primary immune response.1 Due to their...
Natural killer (NK) cells are an important part of the innate immune system and are responsible for killing virally infected cells by secreting cytotoxic granules. Activated NK cells also secrete a variety of cytokines that regulate the function...
In the year 2000, Alan J. Heeger, Alan G.MacDiarmid and Hideki Shirakawa were awarded the Nobel Prize for their discovery of conducting polymers. The revolutionary chemistry afforded by these polymers has enabled the creation of BD Horizon Brilliant™ dyes. These are a range of extremely photostable dyes that allow the collection of excitation light and […]
The article by Pieragostino, D et.al describes how flow cytometry can be used to study extracellular vesicles (EV). Several proteomics studies have been performed to identify new biomarkers for multiple sclerosis (MS) in cerebrospinal fluid (CSF). However, as CSF is complex and requires invasive procedures to extract, other biofluids are being considered. Tears are a […]
In conventional flow cytometry sorting, hardware and instrument restrictions permit only one or two-dimensional regions to be used for gating. In addition, these gates can only be applied to measurements made during data collection. Once the data is collected, sophisticated machine learning algorithms present in software packages like FlowJo™ may be used to identify cell […]
The expression of CCR7 (CD197), a chemokine receptor that controls homing to secondary lymphoid organs, allows the differentiation of human memory T cells into functionally different subsets.1 CCR7+ memory cells express lymph-node homing receptors, efficiently stimulate dendritic cells and differentiate into CCR7- effector cells upon secondary stimulation. CCR7- memory cells express receptors for migration to […]
Chronic Lymphoid Leukemia (CLL), the most common form of leukemia in adults, is often characterised by progressive accumulation of incompetent lymphocytes in blood and bone marrow. Recent bulk RNA studies suggested that there are heterogeneities in CLL samples, prompting the need for single-cell RNA sequencing to study the underlying transcriptional profile. This presentation shows how […]
Part 2: Evolution, challenges and the future of single-cell immunology – Christina Chang In this webinar, the second in the series of the BD Multi-Omics user day presentations, Christina Chang talks about how the BD single-cell, end-to-end workflow with informatics helps to increase the experimental power available to researchers. This workflow comprises of sample preparation, […]
Simultaneous single-cell mRNA and epitope mapping of human CD4+ T-cells using the BD Rhapsody™ and the BD™ AbSeq platform “ Currently a senior post-doctoral fellow in John Todd’s lab at the University of Oxford, my primary research aims at understanding how type 1 diabetes-associated genetic variants are functionally associated with disease. Through a combination of […]
Enable discoveries and get to your next breakthrough moment faster! The BD FACSMelody™ offers high-end sorting in an easy-to-use package for any researcher. It has been contributing to the research in cutting-edge areas like stem cell research, multiomics, immunology and cancer research. Here, we present a list of publications in high-impact journals, citing the BD FACSMelody™. […]