By Dr. Wendy Beguelin, Weil Cornell Medical College. During humoral immune response or infections, subsets of B-cells migrate to germinal centres in lymphoid follicles located in the lymph nodes and...
Persistent antigen stimulation of T-cells, typically observed during chronic infections or in tumor microenvironments, results in the loss of antigen-specific CD8+ T-cell effector function. This phenomenon is referred to as...
The past two years have seen the “cell therapy revolution” begin. It now sees the patient’s cells being modified to fight a disease. This revolution brings along unique challenges from discovery through clinical trials to the clinic. An...
Discover a fully automated sample preparation solution The March of 2019 saw the release of a long-missing, fully automated sample preparation solution by BD. The BD FACSDuet™ sample prep system, when fully integrated with the BD FACSLyric™ flow cytometer, provides a complete walkaway sample to answer solution. Through its automation, the BD FACSDuet™ drives higher […]
BD Horizon Brilliant™ Ultraviolet dyes are being reformulated with improved chemistry to allow you to better optimise your flow cytometry experiments. The new formulations have similar emission and excitation properties as before but have the additional advantage of reduced spillover into the BUV395 detector. BUV563, BUV737 and BUV805 have already been reformulated, and BUV496 and […]
The interactions between B cells and T cells within the germinal centres of peripheral lymphoid organs are crucial for the selection of high affinity B cells and development of long-lived memory cells in the context of T-cell dependent immune responses. Chemokine receptors play key roles in the homing, trafficking and retention of B cells […]
As flow cytometrists, our focus is mostly on setting up the instruments and designing experiments with the most appropriate reagent panels. However, an undeniable fact is that our overriding concern should be about the suffering patients, who badly need our expertise and continuous research. To shed light on this aspect, BD organised an event titled […]
At the BD European Training Centre in Belgium, we offer a 4.5-day training course that teaches users how to work with the BD LSRFortessa™, BD LSRFortessa™ X-20, and BD FACSCelesta™. This exciting training program conducted by BD experts teaches users not only how to work with and maintain these machines, but also covers aspects of […]
The efficient and quick isolation of lymphocytes (B-cells, T-cells, NK cells) and monocytes, both commonly called peripheral blood mononuclear cells or PBMCs, is extremely important for scientists and clinicians working in the field of immunology. Compared with the traditional ficoll-gradient method, BD Vacutainer™ CPT tubes offer great advantages by simplifying the process of PBMC isolation. […]
We are proud to announce the creation of the new block featuring the list of BD™ AbSeq antibody conjugates on www.flowcytometry-news.com. Unlike in conventional flow cytometry, BD™ AbSeq antibodies are conjugated not to dyes but to oligonucleotides. The BD™ AbSeq can be integrated with the BD Rhapsody™ system to enable an efficient workflow that allows researchers to […]
Use of BD Rhapsody™ system for whole transcriptome analysis We are proud to present the first results of a study published in Nature Methods, in which the BD Rhapsody™ system was used for whole transcriptome analysis. In this publication, transcriptional time course, immunocytochemistry and single-cell analyses are used to demonstrate that the derivation of hCS […]