Part 4 – Single-cell expression profiling of mesenchymal stromal cells: Initial experience using BD Rhapsody™ In the fourth and final seminar from the BD Multi-Omics user meeting, Dr. Alison Devonshire...
Multicolour flow cytometry is possibly the most powerful tool for identifying, characterising and phenotyping cells. Dyes are a very important component of multicolour flow cytometry. The brightness and high performance of BD Horizon™ Brilliant dyes have led to...
The Multi-Omics technology delivered by BD is designed to provide an end-to-end, complete workflow for the analysis of single cells. From sample preparation to data analysis, this unique workflow increases experimental capabilities for researchers by enabling protein profiling in addition to gene expression analysis in isolated single cells. Protein expression can be estimated using conjugated […]
In the third webinar in the series of BD Multi-Omics user day presentations, Miha Kosmac of Kymab, Cambridge, UK presents how the BD Rhapsody™ system was used to successfully perform translational immunotyping of matched PBMC and tumor cells. The data presented shows that patterns of inducible co-stimulator (ICOS) expression on different immune cells in these […]
Dendritic cells (DCs), a vital part of the innate and adaptive immune system, are antigen-sensing and presenting cells uniquely possessing the ability to induce primary immune response.1 Due to their important role in the immune system, there is great interest in studying DCs to develop therapies for cancer, chronic infections, autoimmune diseases and for transplantation […]
Natural killer (NK) cells are an important part of the innate immune system and are responsible for killing virally infected cells by secreting cytotoxic granules. Activated NK cells also secrete a variety of cytokines that regulate the function of other cells in the immune system. NK cells are tightly regulated by stimulatory and inhibitory receptors […]
In the year 2000, Alan J. Heeger, Alan G.MacDiarmid and Hideki Shirakawa were awarded the Nobel Prize for their discovery of conducting polymers. The revolutionary chemistry afforded by these polymers has enabled the creation of BD Horizon Brilliant™ dyes. These are a range of extremely photostable dyes that allow the collection of excitation light and […]
The article by Pieragostino, D et.al describes how flow cytometry can be used to study extracellular vesicles (EV). Several proteomics studies have been performed to identify new biomarkers for multiple sclerosis (MS) in cerebrospinal fluid (CSF). However, as CSF is complex and requires invasive procedures to extract, other biofluids are being considered. Tears are a […]
In conventional flow cytometry sorting, hardware and instrument restrictions permit only one or two-dimensional regions to be used for gating. In addition, these gates can only be applied to measurements made during data collection. Once the data is collected, sophisticated machine learning algorithms present in software packages like FlowJo™ may be used to identify cell […]
The expression of CCR7 (CD197), a chemokine receptor that controls homing to secondary lymphoid organs, allows the differentiation of human memory T cells into functionally different subsets.1 CCR7+ memory cells express lymph-node homing receptors, efficiently stimulate dendritic cells and differentiate into CCR7- effector cells upon secondary stimulation. CCR7- memory cells express receptors for migration to […]