Driving Deeper Discovery with High Parameter Flow Cytometry Over the past ten years, flow cytometry has advanced significantly. Cell surface phenotyping (with 4 or 8 markers) has evolved to allow much more complex multi-parameter cellular assessments that incorporate signal transduction pathways (Phosflow), transcriptional nodes (eg. FoxP3, STAT) and peptide-loaded class restricted multimers. Collectively, these developments […]
BD’s flow cytometer FACSymphonyTM S6 was showcased during April’s CYTO 2018 in Prague. Visitors to our booth discovered how this high performance, flexible and reliable cell sorter benefits from our 45 years of experience in cutting-edge diagnostics. Compatible with BD FACSDivaTM software, it can be configured with up to 9 spatially separated lasers, with assays […]
BD’s new single-cell analyser Rhapsody™ is already generating news. The UK National Measurement Laboratory ‘s Molecular and Cell Biology team is talking about the exciting experiments planned for their new Rhapsody™ analyser. Science Leader Alison Devonshire says, ‘This new technology will […] support our activities […],for example for stem cell based therapies or understanding the heterogeneity of […]
BD recently expanded manufacturing and global supply operations, with the inauguration of the new RUO reagent manufacturing facility in Tatabánya, Hungary. Local scientists who attended the October 2017 Opening Celebrations were impressed by the well designed labs and the latest, high quality equipment. ‘The Quality Assurance Processes were impressive’ said Timea Berki MD, PhD . To a bright future – Egészségére !
An important factor that must be considered for proper panel design is the sensitivity of the reagent. Sensitivity is influenced by the brightness of the fluorochrome and the data spread due to the presence of other antibody conjugates attached to the same cell. Not all fluorochromes have the same sensitivity due to differing brightness. Sensitivity is also impacted by the expression level of the markers being studied. Markers, or antigens, can be classified as primary, secondary or tertiary, depending on their levels of expression. Many candidate antibody-conjugate panels may need to be evaluated to determine which one optimally stains all the antigens under study. This is also important to determine which antibody conjugates reduce the sensitivity. Single stain controls to evaluate and correct for compensation must also be performed. Multicolor experiments can be frustrating but using the right panel can give wonderful results and it is important to invest time and effort into this.
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