Delineation of functional Treg subsets using cell surface marker expression

Regulatory T cells or Tregs are CD4 T cells that are essential for maintaining peripheral tolerance, preventing autoimmunity and limiting chronic inflammatory diseases.

Naturally occurring Tregs were first characterized within the CD4+CD25+ T cell populations. Tregs express the transcription factor Foxp3, which is essential for their function. Over the years, specific Treg subsets have been identified, supported by the discovery of a growing number of cell surface markers. One such marker highlighted in this application note is neuropilin-1 (Nrp-1 or CD304), which distinguishes natural Tregs from induced Tregs in mice. Another  surface marker described here is ectonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39), which mediates adenosine-triggered immunosuppressive effects in both mice and humans. Both markers are potential immune checkpoint targets in cancer immunotherapy.

This application note describes a study in which a 29-colour panel was used to identify Treg subsets in cells from C57BL/6 mouse spleens. The data was acquired on a BD FACSymphony™ A5 Cell Analyser and analysed using FlowJo™ v10.71.

Learn more about the annotation of cell populations and the expression of the markers by downloading the application note here.

This application note is not peer-reviewed.
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