Harmony Seminar – An optimised method for accurate spread measurement and its use in panel design

Do you run multicolour flow cytometry experiments with more than 10 colours? Do you use tools like Spillover Spread Matrix (SSM) to predict spread and design multicolour panels? Do you find discrepancies between predicted and measured spread, resulting in sub-optimal resolution?

If the answer to these questions is yes, then this webinar is for you!

While designing multicolour flow cytometry panels, it important to assess fluorescence spillover, to prevent or minimize loss of resolution due to the spreading. The SSM was developed as a tool to monitor and compare instrument performance over time, especially in the setting of experiments standardised or calibrated across different instruments.1

The SSM is independent of fluorochrome brightness. While this feature is important for the comparison of instruments, it may lead to inaccurate spread prediction and sub-optimal panel design.

In this webinar, Bob Balderas, Distinguished BD Biosciences fellow, VP Biological Sciences, VP Market Development, and Mirko Corselli, Global Scientific Content Manager, introduce a new, optimised method for a more accurate spread assessment and explain how it can aid in panel design.

Watch the recorded webinar here.

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1 Nguyen R, Perfetto S, Mahnke YD, et.al . Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design. Cytometry A. 2013 Mar;83(3):306-15. doi: 10.1002/cyto.a.22251
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