Leveraging the power of high-parameter cell sorting and single-cell multi-omics to profile intra-tumoral immune cells in a model of B cell lymphoma

Scientific Publication

The pathogenesis of B cell malignancies is a dynamic process The pathogenesis of B cell malignancies is a dynamic process involving reciprocal interactions between malignant cells and surrounding components in the tumour microenvironment. In the case of certain B cell lymphomas, neoplastic cells migrate to lymphoid sites and prompt the infiltration of non-malignant immune cells. […]

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Assessing tumour-driven perturbation during haematopoiesis in a melanoma mouse model through single-cell multi-omic analysis

Scientific Publication

Haematopoiesis and tumour growth During haematopoiesis, multilineage blood cells are generated successively from a series of lineage-restricted progenitor cells, in turn, derived from haematopoietic stem cells. Haematopoietic stem and progenitor cells (HSPCs) are usually localised in the bone marrow niche during the lifetime but extramedullary haematopoiesis can occur in organs like the spleen during pathophysiological […]

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Autophagy in viral replication : SKP2 mediated BECN1 ubiquitination

Scientific Publication

SKP2 mediated BECN1 ubiquitination, its role in autophagy, and how its inhibition reduces MERS-Coronavirus replication   Autophagy is an important mechanism used by host cells to counter viral infections. In this process, double-membrane vesicles called autophagosomes deliver viral cargo to the lysosomes for degradation. Autophagy also coordinates the presentation of the virus-derived antigens to T […]

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Single-cell genomic approaches for developing the next generation of immunotherapies

Scientific Publication

In recent times, developments in genomics, proteomics and single-cell technologies, coupled with advances in allied areas like computing and antibody engineering have enabled significant progress in drug development. Current drug development platforms, however, take only simplified cellular models or molecular readouts into account at the functional level. This makes it difficult to evaluate the effects […]

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High-dimensional flow cytometry in COVID-19 research

Scientific Publication

Targets of T cell responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals The rapid spread of COVID-19 infections all over the world and the need to develop a vaccine has necessitated the understanding of the adaptive immune response to the SARS-CoV-2 virus. There is an urgent requirement to evaluate the response […]

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Targeted Multi-Omics analysis and high-dimensional flow cytometry to measure protein expression and low-abundance transcripts at the single-cell level.

Scientific Publication

In this article published in Cell Reports, Mair et. al report a study on the use of targeted Multi-Omics to study immune-cell heterogeneity at a low sequencing depth. Working with sorted pan CD45+ live cells derived from cryopreserved PBMCs, the authors used a high-throughput (>104 single cells) approach in which they captured the cells in […]

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Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway

Scientific Publication

Long term immunoprotection or naturally acquired immunity is conferred by memory T cells and is initiated either upon the first exposure to a pathogen or vaccination. The maintenance of this immunological memory for long durations requires the preservation of T cell stemness and the ability of memory T cell compartments to alternate between an active […]

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The use of high-dimensional flow cytometry in the study of Tregs in tumor microenvironments and immune suppression

Scientific Publication

In physiology, regulatory CD4+ T cells or Tregs, are responsible for maintaining immune homeostasis by inhibiting effector T cells, thereby preventing autoimmunity. Tregs do this in different ways, for example, by expressing cell-surface inhibitors, producing inhibitory cytokines, depletion of IL-2, purine-mediated suppression of CD39 dependent conversion of ATP or direct cytotoxicity.   However, in tumor microenvironments, […]

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High-dimensional flow cytometric analysis of human B-cell populations

Scientific Publication

B-cell development is initiated in the fetal liver and neo-natal bone marrow and continues in the peripheral lymphoid organs upon exposure to antigens. The various stages of the development of B cells are associated with the several cell-surface markers that are regulated throughout the process.  As with all differentiation processes associated with a large number […]

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Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1

Scientific Publication

The authors of this article (Hasegawa, et.al, Nat.Immun 2019) have studied the formation of osteoclasts, which play a crucial role in arthritic bone erosion. They have developed an original protocol to isolate the inflamed synovium from arthritic mice and have identified specific  macrophages called arthritis-associated osteoclastogenic macrophages (AtoMs) as osteoclast precursors in arthritic joints. AtoMs […]

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