Scientific Publication
Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1
Scientific PublicationThe authors of this article (Hasegawa, et.al, Nat.Immun 2019) have studied the formation of osteoclasts, which play a crucial role in arthritic bone erosion. They have developed an original protocol to isolate the inflamed synovium from arthritic mice and have identified specific macrophages called arthritis-associated osteoclastogenic macrophages (AtoMs) as osteoclast precursors in arthritic joints. AtoMs […]
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Prostaglandin E2 stimulates the expansion of regulatory haematopoetic stem and progenitor cells in Type 1 diabetes
Scientific PublicationThe transplant of Haematopoetic Stem and Progenitor cells (HSPC) has been employed in the treatment of haematological malignancies. However, studies have shown that HSPCs also possess immunoregulatory properties, making them potentially attractive alternatives to immunosuppressive drugs for the treatment of autoimmune diseases. Clinical trials involving transplantation of autologous haematopoetic stem and progenitor cells in new […]
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Evaluating expression patterns of multiple inhibitory and stimulatory receptors on natural killer cells using flow cytometry
Scientific PublicationNatural killer (NK) cells are an important part of the innate immune system and are responsible for killing virally infected cells by secreting cytotoxic granules. Activated NK cells also secrete a variety of cytokines that regulate the function of other cells in the immune system. NK cells are tightly regulated by stimulatory and inhibitory receptors […]
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Proteomics characterization of extracellular vesicles sorted by flow cytometry
Scientific PublicationThe article by Pieragostino, D et.al describes how flow cytometry can be used to study extracellular vesicles (EV). Several proteomics studies have been performed to identify new biomarkers for multiple sclerosis (MS) in cerebrospinal fluid (CSF). However, as CSF is complex and requires invasive procedures to extract, other biofluids are being considered. Tears are a […]
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Evaluating the expression patterns of multiple inhibitory receptors associated with T-cell exhaustion using multicolor flow cytometry
Scientific PublicationEvaluating the expression patterns of multiple inhibitory receptors Persistent antigen stimulation of T-cells, typically observed during chronic infections or in tumor microenvironments, results in the loss of antigen-specific CD8+ T-cell effector function. This phenomenon is referred to as T-cell exhaustion and is characterised by the progressive loss of function and proliferative capacity, eventually resulting in […]
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The first publication featuring the BD Rhapsody™ system – Reliability of human cortical organoid generation
Scientific PublicationUse of BD Rhapsody™ system for whole transcriptome analysis We are proud to present the first results of a study published in Nature Methods, in which the BD Rhapsody™ system was used for whole transcriptome analysis. In this publication, transcriptional time course, immunocytochemistry and single-cell analyses are used to demonstrate that the derivation of hCS […]
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Revealing rare stem cells with single-cell analysis
Scientific PublicationNew single-cell Multi-Omics techniques are being developed that can enable researchers to measure both mRNA and protein expression simultaneously in a single cell, revealing more information than historically used techniques. This new white paper reviews current single-cell techniques used in stem cell research and how Multi-Omics has the potential to reveal new sub-populations of stem […]
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A multi-omics approach to investigate the heterogeneity of memory T cells
Scientific PublicationThe post-thymic activation and development of T cells involves a heterogeneous population of cells at different stages of differentiation, including naïve, stem cell memory, central memory, effector memory and terminal effector T cells. These different T-cell subsets are themselves highly heterogeneous, and their identification and characterization require not one or a few but, rather, a […]
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How MAIT cells influence our immune Defence
Scientific PublicationScientists already know that MAIT ( Mucosal-associated invariant T) cells defend us against invading bacteria and fungi. Recently, a team of UK-based researchers discovered how MAIT cells also perform another of their vital functions: orchestrating the activity of other important cells in the immune system, such as dendritic cells (DCs). As you will see, Flow […]
Continue reading 15minFlow cytometry: at the frontier of biological research
Scientific PublicationIn the study of rare cell types, Flow Cytometry is proposed to overcome the limitations of ELISA. By providing both phenotyping and functional data in the same assay, Flow Cytometry is well suited for investigating rare cell populations, especially cytokine producing cell subsets in an heterogeneous sample. Combined with the flexibility of Optibuild reagents for panel design, BD’s FACSCelesta™ allows you to perform advanced analysis of rare cells such as DCs and Tregs.
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