Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway

Scientific Publication

Long term immunoprotection or naturally acquired immunity is conferred by memory T cells and is initiated either upon the first exposure to a pathogen or vaccination. The maintenance of this immunological memory for long durations requires the preservation of T cell stemness and the ability of memory T cell compartments to alternate between an active […]

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The use of high-dimensional flow cytometry in the study of Tregs in tumor microenvironments and immune suppression

Scientific Publication

In physiology, regulatory CD4+ T cells or Tregs, are responsible for maintaining immune homeostasis by inhibiting effector T cells, thereby preventing autoimmunity. Tregs do this in different ways, for example, by expressing cell-surface inhibitors, producing inhibitory cytokines, depletion of IL-2, purine-mediated suppression of CD39 dependent conversion of ATP or direct cytotoxicity.   However, in tumor microenvironments, […]

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High-dimensional flow cytometric analysis of human B-cell populations

Scientific Publication

B-cell development is initiated in the fetal liver and neo-natal bone marrow and continues in the peripheral lymphoid organs upon exposure to antigens. The various stages of the development of B cells are associated with the several cell-surface markers that are regulated throughout the process.  As with all differentiation processes associated with a large number […]

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Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1

Scientific Publication

The authors of this article (Hasegawa, et.al, Nat.Immun 2019) have studied the formation of osteoclasts, which play a crucial role in arthritic bone erosion. They have developed an original protocol to isolate the inflamed synovium from arthritic mice and have identified specific  macrophages called arthritis-associated osteoclastogenic macrophages (AtoMs) as osteoclast precursors in arthritic joints. AtoMs […]

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Prostaglandin E2 stimulates the expansion of regulatory haematopoetic stem and progenitor cells in Type 1 diabetes

Scientific Publication

The transplant of Haematopoetic Stem and Progenitor cells (HSPC) has been employed in the treatment of haematological malignancies. However, studies have shown that HSPCs also possess immunoregulatory properties, making them potentially attractive alternatives to immunosuppressive drugs for the treatment of  autoimmune diseases.  Clinical trials involving transplantation of autologous haematopoetic stem and progenitor cells in new […]

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Evaluating expression patterns of multiple inhibitory and stimulatory receptors on natural killer cells using flow cytometry

Scientific Publication

Natural killer (NK) cells are an important part of the innate immune system and are responsible for killing virally infected cells by secreting cytotoxic granules. Activated NK cells also secrete a variety of cytokines that regulate the function of other cells in the immune system. NK cells are tightly regulated by stimulatory and inhibitory receptors […]

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Proteomics characterization of extracellular vesicles sorted by flow cytometry

Scientific Publication

The article by Pieragostino, D et.al describes how flow cytometry can be used to study extracellular vesicles (EV). Several proteomics studies have been performed to identify new biomarkers for multiple sclerosis (MS) in cerebrospinal fluid (CSF). However, as CSF is complex and requires invasive procedures to extract, other biofluids are being considered. Tears are a […]

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Evaluating the expression patterns of multiple inhibitory receptors associated with T-cell exhaustion using multicolor flow cytometry

Scientific Publication

Evaluating the expression patterns of multiple inhibitory receptors Persistent antigen stimulation of T-cells, typically observed during chronic infections or in tumor microenvironments, results in the loss of antigen-specific CD8+ T-cell effector function. This phenomenon is referred to as T-cell exhaustion and is characterised by the progressive loss of function and proliferative capacity, eventually resulting in […]

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The first publication featuring the BD Rhapsody™ system – Reliability of human cortical organoid generation

Scientific Publication

Use of BD Rhapsody™ system for whole transcriptome analysis We are proud to present the first results of a study published in Nature Methods, in which the BD Rhapsody™ system was used for whole transcriptome analysis. In this publication, transcriptional time course, immunocytochemistry and single-cell analyses are used to demonstrate that the derivation of hCS […]

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Revealing rare stem cells with single-cell analysis

Scientific Publication

New single-cell Multi-Omics techniques are being developed that can enable researchers to measure both mRNA and protein expression simultaneously in a single cell, revealing more information than historically used techniques. This new white paper reviews current single-cell techniques used in stem cell research and how Multi-Omics has the potential to reveal new sub-populations of stem […]

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