BD™ AbSeq protocol using the nano-well cartridge-based BD Rhapsody™ platform

Scientific Publication

The BD™ AbSeq oligonucleotide-conjugated antibodies The BD™ AbSeq oligonucleotide-conjugated antibodies, when incorporated into a single-cell mRNA-sequencing experiment, can yield combined transcript and protein expression data. Such data can provide vital insights into cellular processes and yield insightful information about gene expression. This article by Erickson, et.al provides a detailed experimental protocol for the combined analysis […]

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CRISPR screens using targeted single-cell RNA sequencing

Scientific Publication

Cell perturbation using the CRISPR technique Earlier on www.flowcytometry-news.com, we featured an article on the use of the BD Rhapsody™ system to perform highly scalable and more sensitive CRISPR screens. In cell perturbation using the CRISPR technique, single guide RNAs (sgRNAs) are used to guide the endonuclease Cas9 to create a targeted double-strand break, the […]

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OMIP-068: High-dimensional characterization of global and antigen-specific B cells in chronic infection

Scientific Publication

OMIP-068: High-dimensional characterization In this OMIP (Optimised Multicolour Immunophenotyping Panel), the authors describe a 24-color panel to characterise antigen-specific B cells and for the precise delineation of B cell subsets in chronic infection.1 B cells play an important role in infection response through the production of antibodies, antigen presentation and secretion of cytokines. The antibodies […]

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OMIP-070: NKp46-based 27-color phenotyping to define natural killer cells isolated from human tumor tissues

Scientific Publication

Optimised Multicolour Immunophenotyping Panel In this OMIP (Optimised Multicolour Immunophenotyping Panel), the authors describe a 27-color phenotyping panel to study NK, or Natural Killer cells.1 The interest in NK cells began in the 1970s when it was discovered that they possess the ability to destroy tumor cells, and could play a role in controlling malignancies […]

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Leveraging the power of high-parameter cell sorting and single-cell multi-omics to profile intra-tumoral immune cells in a model of B cell lymphoma

Scientific Publication

The pathogenesis of B cell malignancies is a dynamic process The pathogenesis of B cell malignancies is a dynamic process involving reciprocal interactions between malignant cells and surrounding components in the tumour microenvironment. In the case of certain B cell lymphomas, neoplastic cells migrate to lymphoid sites and prompt the infiltration of non-malignant immune cells. […]

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Assessing tumour-driven perturbation during haematopoiesis in a melanoma mouse model through single-cell multi-omic analysis

Scientific Publication

Haematopoiesis and tumour growth During haematopoiesis, multilineage blood cells are generated successively from a series of lineage-restricted progenitor cells, in turn, derived from haematopoietic stem cells. Haematopoietic stem and progenitor cells (HSPCs) are usually localised in the bone marrow niche during the lifetime but extramedullary haematopoiesis can occur in organs like the spleen during pathophysiological […]

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Autophagy in viral replication : SKP2 mediated BECN1 ubiquitination

Scientific Publication

SKP2 mediated BECN1 ubiquitination, its role in autophagy, and how its inhibition reduces MERS-Coronavirus replication   Autophagy is an important mechanism used by host cells to counter viral infections. In this process, double-membrane vesicles called autophagosomes deliver viral cargo to the lysosomes for degradation. Autophagy also coordinates the presentation of the virus-derived antigens to T […]

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Single-cell genomic approaches for developing the next generation of immunotherapies

Scientific Publication

In recent times, developments in genomics, proteomics and single-cell technologies, coupled with advances in allied areas like computing and antibody engineering have enabled significant progress in drug development. Current drug development platforms, however, take only simplified cellular models or molecular readouts into account at the functional level. This makes it difficult to evaluate the effects […]

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High-dimensional flow cytometry in COVID-19 research

Scientific Publication

Targets of T cell responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals The rapid spread of COVID-19 infections all over the world and the need to develop a vaccine has necessitated the understanding of the adaptive immune response to the SARS-CoV-2 virus. There is an urgent requirement to evaluate the response […]

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Targeted Multi-Omics analysis and high-dimensional flow cytometry to measure protein expression and low-abundance transcripts at the single-cell level.

Scientific Publication

In this article published in Cell Reports, Mair et. al report a study on the use of targeted Multi-Omics to study immune-cell heterogeneity at a low sequencing depth. Working with sorted pan CD45+ live cells derived from cryopreserved PBMCs, the authors used a high-throughput (>104 single cells) approach in which they captured the cells in […]

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