Persistent antigen stimulation of T-cells, typically observed during chronic infections or in tumor microenvironments, results in the loss of antigen-specific CD8+ T-cell effector function. This phenomenon is referred to as T-cell exhaustion and is characterised by the progressive loss of function and proliferative capacity, eventually resulting in clonal elimination.
A typical feature of T-cell exhaustion is the increased expression of inhibitory receptors, which attenuate T-cell activation through a variety of processes. Studies on the two well known inhibitory receptors, CTLA-4 and PD-1, have shown that their inhibition reverses T-cell exhaustion, diminishes tumor growth and improves survival rates. However, the inhibition of these two receptors has been seen to be ineffective in many patients due to the immune system developing additional, non-redundant inhibitory mechanisms.
This white paper presents the analysis of six T-cell inhibitory receptors in fresh and in-vitro simulated T cells using 12-colour flow cytometry. It establishes that 12-colour flow cytometry is an effective tool for studying complex, heterogenous expression patterns of T-cells expressing multiple inhibitors.
Click here to download the white paper.