Proteomics characterization of extracellular vesicles sorted by flow cytometry

The article by Pieragostino, D et.al describes how flow cytometry can be used to study extracellular vesicles (EV). Several proteomics studies have been performed to identify new biomarkers for multiple sclerosis (MS) in cerebrospinal fluid (CSF). However, as CSF is complex and requires invasive procedures to extract, other biofluids are being considered. Tears are a potentially useful candidate as biofluid as they are associated with the central nervous system and therefore, contain EVs from brain cells that can be used in the search for MS biomarkers.

The authors of the paper found microglia and neural-derived EVs in tears for the first time. They used a flow cytometer to purify EVs from untouched CSF and tears, from both healthy and MS patients, and detected similar proteins in both using shotgun proteomic analysis. Their results suggest an EV-mediated crosstalk between CSF and tears, and provide new options for biomarker research in MS.

The study has been published in the Journal of Proteomics. Click <here> to access the article through the journal website.

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