The organoid cell atlas

Scientific Publication

Organoids are three-dimensional assemblies of cultured cells that reiterate important aspects of organ development and provide an insight into biological function. They are viable, in vitro models of human physiology and can be used for interventional studies that are not possible in human subjects. Organoids reflect human biology and allow for genetic manipulation and complex […]

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Multi-omics characterization of T-cell populations at the single-cell level utilizing sensitive dCODE Dextramer® and the BD® AbSeq on the BD Rhapsody™ Single-Cell Analysis System

News & innovations, The Cytohub

Why identifying antigen-specific epitopes and their cognate T-cell receptors is important In immunology, identifying antigen-specific epitopes and their cognate T-cell receptors can yield useful insights into the functioning of T-cells and can provide vital information for vaccine development. dCODE Dextramer® (RiO) reagents are DNA barcoded MHC Dextramer® products compatible with the BD RhapsodyTM Single-Cell Analysis System. Each […]

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Visual metrics in BD Multi-Omics: Your key to finding the impossible!

The Cytohub

It has always been difficult to find the impossible, but what makes it so? To unravel the secrets of single-cells it is important to start the experiment with viable and live cells. The BD Multi-Omics workflow enables this using visual metrics, providing you with accurate information about cell counts and viability along the entire process. […]

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CRISPR screens using targeted single-cell RNA sequencing

Scientific Publication

Cell perturbation using the CRISPR technique Earlier on www.flowcytometry-news.com, we featured an article on the use of the BD Rhapsody™ system to perform highly scalable and more sensitive CRISPR screens. In cell perturbation using the CRISPR technique, single guide RNAs (sgRNAs) are used to guide the endonuclease Cas9 to create a targeted double-strand break, the […]

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Leveraging the power of high-parameter cell sorting and single-cell multi-omics to profile intra-tumoral immune cells in a model of B cell lymphoma

Scientific Publication

The pathogenesis of B cell malignancies is a dynamic process The pathogenesis of B cell malignancies is a dynamic process involving reciprocal interactions between malignant cells and surrounding components in the tumour microenvironment. In the case of certain B cell lymphomas, neoplastic cells migrate to lymphoid sites and prompt the infiltration of non-malignant immune cells. […]

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