Multicolour flow cytometry panels for COVID-19 research!

The Cytohub

Enabling your research into the immune response to COVID-19 There is now a pressing need to unravel the biological mechanisms underlying COVID-19 infections, as the pandemic continues to spread all around the world. Research studies have shown that there is an alteration in the composition of immune cells and dysfunctional immune response in COVID-19 patients. […]

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Training: The BD FACSAria™ virtual class

The Cytohub

The BD FACSAria™ family of cell sorters The BD FACSAria™ family of cell sorters have established a reputation for superior multicolour performance and legendary ease of use. They have opened the field of cell sorting to a broader audience of researchers and a wider range of applications. They feature precisely integrated fluidics and optical systems […]

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The BD Customs program – A new site for custom reagents for flow cytometry

The Cytohub

BD now has a new custom reagent website. BD now has a new custom reagent website. It combines our expertise in the domains of flow cytometry, fluorochrome science and immunology to deliver custom solutions for your needs. For an immunologist performing flow experiments, our website offers many exciting possibilities for customisation. Information is available on […]

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Assessing immunological abnormalities in COVID-19 disease by enumerating CD4 and CD8 T cell subsets

The Cytohub

T cell subsets in COVID-19 immune response CD4 and CD8 T cells are important for triggering an immune response to bacterial and viral infections. Complete blood counts (CBCs) are often carried out in patients suffering from infections. However, while CBC tests can detect lymphopenia in blood, they cannot measure the specific lymphocyte populations that are […]

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Webinar: Single-cell multi-omics identifies unstable regulatory T-cell profiles

The Cytohub

Webinar by Prof. Susan Schlenner, University of Leuven, Belgium Regulatory T cells (Tregs) are a subset of CD4+ T cells that possess immunosuppressive functions.  They play a key role in human health as their malfunction results in allergies and auto-inflammatory disorders. Loss of regulatory T cells results in a rare autoimmune polyendocrine syndrome called IPEX, […]

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Now more flexibility in panel design with the BD Horizon™ Red 718

The Cytohub

BD Horizon™ R718 The BD Horizon™ R718, developed exclusively by BD Biosciences, is a small-molecule dye that is excited by the red laser (628 or 640 nm) and has an emission maximum of 718 nm. It can be used as a replacement for Alexa-Fluor® 700 or APC-R700 without the need to change the instrument settings, […]

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Highly scalable and more sensitive CRISPR screens using the BD Rhapsody™ System

The Cytohub

A well-understood technique for the targeted disruption of genes across a wide range of cell types is the use of the CRISPR/Cas9 method. In this, a single guide RNA (sgRNA) strand is used to guide the Cas9 endonuclease to create a double-strand break at a site that is complementary to the sgRNA. The resulting frameshift […]

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Met-Flow: A strategy for single-cell metabolic analysis

News & Event, The Cytohub

Featuring World-Renowned Flow Cytometry Expert:  Dr. John Connolly Wednesday, October 21, 2020 1 p.m. SGT A complex interaction of anabolic and catabolic metabolism A complex interaction of anabolic and catabolic metabolism underpins the ability of leukocytes to mount an immune response. Their capacity to respond to changing environments by metabolic reprogramming is crucial to effector function. […]

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Webinar: Single-cell Multiomics analysis of T-cell exhaustion

The Cytohub

A key step in the clinical production of CAR T cells is the expansion of engineered T cells. To generate enough cells for viable adoptive cell therapy, cells must be robustly stimulated, which raises the risk of inducing T-cell exhaustion and reducing therapeutic efficacy. We sought to answer fundamental questions about the impact of in […]

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BD Harmony Webinars by Bob Balderas, BD Biosciences

The Cytohub

Learning panel design Panel design is one of the most crucial parts of a flow-cytometry experiment. It involves the selection of the right combination of marker and fluorochrome for a given population of cells under study. A badly designed panel, comprising of improperly selected fluorochromes results in overlapping of emission spectra, leading to loss of […]

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