Fluorescence assisted cell-sorting in a oncohematology laboratory

The Cytohub

FACS or fluorescence assisted cell sorting, is an efficient technique to sort and select specific cell types from mixed populations. The authors of this poster have carried out molecular biology experiments like fluorescent in-situ hybridization (FISH), TCR re-arrangement and allele specific oligonucleotide tests on bone marrow samples taken from multiple myeloma, B-ALL, B-LS and mastocytosis […]

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BD OptiBuild™ – Obtaining the best results by titrating

The Cytohub

The BD OptiBuild™ portfolio offers a wide range of dye-antibody combinations, allowing researchers to design sophisticated panels with minimum delay. However, once a panel is designed, the optimal concentration of the reagent for your specific sample needs to be established. To get the best results from a 50 µg reagent vial, a titration series of […]

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BD Horizon™ Dri Chroma – The Brilliance of a complete panel in a tube

The Cytohub

BD Horizon™ Dri Chroma is a new technology that allows up to 16 reagents, including up to 5 BD Horizon™ Brilliant reagents, to be cocktailed and supplied in a dried down format in a single tube. This means greater workflow efficiency and stability versus liquid reagents. The BD Horizon™ Dri Chroma multicolour panels also show […]

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Exploring tumor heterogeneity of chronic lymphocytic leukemia (CLL) using a unique workflow.

The Cytohub

CLL or chronic lymphocytic leukemia is the most common form of leukemia in adults. Understanding the heterogeneity of CLL tumor cells is important for the development of treatments and many bulk RNA-sequencing studies have provided insights into CLL pathogenesis. However, the complex mixture of cells used in such studies has failed to accurately elucidate the […]

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BD FACSMelody™ and BD Rhapsody™: Flow cytometry and Multi-Omics walking hand-in-hand down the aisle of single-cell analysis!

The Cytohub

Are you wishing for new insights into cell heterogeneity that phenotyping can’t reveal? What if you could combine flow cytometry and Multi-Omics in a smooth workflow to gain an unprecedented view into single cells? The combination of the BD FACSMelody™ cell sorter and the BD Rhapsody™ single-cell analysis system enables you to achieve just these. […]

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Whole transcriptome analysis (WTA) of a single cell – Now made easy.

The Cytohub

Unraveling the whole transcriptome of a single cell is now easier with the BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit. First, the lysis, capture of mRNAs using the bead-based retrieval mechanism of the BD Rhapsody™ and cDNA library preparation are carried out. Following this, the BD™ WTA Amplification Kit is used to generate whole […]

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Simultaneous analysis of mRNA and proteins in mouse immune cells using the BD™ Mouse Single-cell multiplexing kit and the BD™ AbSeq assay on the BD Rhapsody™ system

The Cytohub

The authors of this poster have studied the effects of obesity on immune cell populations in mice. They used cells isolated from epididymal fat, thymus, spleen and bone marrow from mice placed on high fat (HFD) and control diets. DNA-barcoded universal antibodies from the BD™ Mouse Single-cell Multiplexing kit allowed them to combine 8 samples […]

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Custom reagents for more effective panel design with BD Optibuild™

The Cytohub

Worried that the dye-antibody combination for a particular marker in your panel is not readily available? With BD OptiBuild™, you don’t need to redesign it. Just check out the BD Optibuild™ range, which may have the antibody conjugate you need. With our ever-expanding range of clones and dyes, we at BD are able to offer […]

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Born to transform – The BD Multi-Omics Alliance

The Cytohub

We are pleased to announce the formation of the “BD Multi-Omics Alliance”, consisting of a balanced team of 7 renowned academic experts from across Europe supported by BD. Driven by the experts’ vision and their common belief that a true understanding of complex diseases or biological processes requires the simultaneous evaluation of protein and mRNA […]

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BD Single-Cell Multi-Omics user meeting seminar series. Part 4 – Single-cell expression…

The Cytohub

Part 4 – Single-cell expression profiling of mesenchymal stromal cells: Initial experience using BD Rhapsody™ In the fourth and final seminar from the BD Multi-Omics user meeting, Dr. Alison Devonshire from the National Measurement Laboratory, United Kingdom, talks about the use of the BD Multi-Omics technology for the expression profiling of mesenchymal stromal cells (MSC). […]

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