The Cytohub
BD single-cell multi-omics user meeting seminar series Part 3: Translational immunophenotyping using BD Rhapsody™
The CytohubIn the third webinar in the series of BD Multi-Omics user day presentations, Miha Kosmac of Kymab, Cambridge, UK presents how the BD Rhapsody™ system was used to successfully perform translational immunotyping of matched PBMC and tumor cells. The data presented shows that patterns of inducible co-stimulator (ICOS) expression on different immune cells in these […]
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Overview of Dendritic Cell Development
The CytohubDendritic cells (DCs), a vital part of the innate and adaptive immune system, are antigen-sensing and presenting cells uniquely possessing the ability to induce primary immune response.1 Due to their important role in the immune system, there is great interest in studying DCs to develop therapies for cancer, chronic infections, autoimmune diseases and for transplantation […]
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BD Horizon Brilliant™ dyes – Giving your research the Brilliant edge
The CytohubIn the year 2000, Alan J. Heeger, Alan G.MacDiarmid and Hideki Shirakawa were awarded the Nobel Prize for their discovery of conducting polymers. The revolutionary chemistry afforded by these polymers has enabled the creation of BD Horizon Brilliant™ dyes. These are a range of extremely photostable dyes that allow the collection of excitation light and […]
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Computational sorting with HyperFinder™
The CytohubIn conventional flow cytometry sorting, hardware and instrument restrictions permit only one or two-dimensional regions to be used for gating. In addition, these gates can only be applied to measurements made during data collection. Once the data is collected, sophisticated machine learning algorithms present in software packages like FlowJo™ may be used to identify cell […]
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The new and improved anti-human CCR7 antibody
The CytohubThe expression of CCR7 (CD197), a chemokine receptor that controls homing to secondary lymphoid organs, allows the differentiation of human memory T cells into functionally different subsets.1 CCR7+ memory cells express lymph-node homing receptors, efficiently stimulate dendritic cells and differentiate into CCR7- effector cells upon secondary stimulation. CCR7- memory cells express receptors for migration to […]
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CLL phenotyping with BD Rhapsody™ Targeted Panels and BD® AbSeq assays
The CytohubChronic Lymphoid Leukemia (CLL), the most common form of leukemia in adults, is often characterised by progressive accumulation of incompetent lymphocytes in blood and bone marrow. Recent bulk RNA studies suggested that there are heterogeneities in CLL samples, prompting the need for single-cell RNA sequencing to study the underlying transcriptional profile. This presentation shows how […]
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BD single-cell multi-omics user meeting seminar series. Part 2 : Christina Chang, PhD
The CytohubPart 2: Evolution, challenges and the future of single-cell immunology – Christina Chang In this webinar, the second in the series of the BD Multi-Omics user day presentations, Christina Chang talks about how the BD single-cell, end-to-end workflow with informatics helps to increase the experimental power available to researchers. This workflow comprises of sample preparation, […]
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BD single-cell multi-omics user meeting seminar series. Part 1 : Dr Ricardo C. Ferreira, University of Oxford
The CytohubSimultaneous single-cell mRNA and epitope mapping of human CD4+ T-cells using the BD Rhapsody™ and the BD™ AbSeq platform “ Currently a senior post-doctoral fellow in John Todd’s lab at the University of Oxford, my primary research aims at understanding how type 1 diabetes-associated genetic variants are functionally associated with disease. Through a combination of […]
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Breakthrough with the BD FACSMelody™ Cell Sorter
The CytohubEnable discoveries and get to your next breakthrough moment faster! The BD FACSMelody™ offers high-end sorting in an easy-to-use package for any researcher. It has been contributing to the research in cutting-edge areas like stem cell research, multiomics, immunology and cancer research. Here, we present a list of publications in high-impact journals, citing the BD FACSMelody™. […]
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Creating the next generation of flow cytometry leaders -BD FACSMelody™ in action in Australia!
The Cytohub“The FACSMelody™ is very simple to use. We can train a user within an hour, even if they have limited flow cytometry experience”, says Dr. Andrea L. Holme, Senior Lecturer for Cytometry at the University of Western Australia, and a happy BD FACSMelody™ user. Watch the video to learn about the work being done by […]
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