The correct choice of the reagents is an important consideration in flow cytometry as the wrong one can result in the loss of resolution in experimental data. In experiments involving the detection of several markers, the choice of the reagent panel is governed by whether the markers are co-expressed or not. In co-expressed markers, the choice of the fluorochrome should be based on the relative expression of the markers, minimizing spectral overlap on these reagents. The principles of panel design and other resources from BD will help you get the best resolution of the populations of interest in your flow cytometry experiments.
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