Multicolour flow cytometry is possibly the most powerful tool for identifying, characterising and phenotyping cells. Dyes are a very important component of multicolour flow cytometry. The brightness and high performance of BD Horizon™ Brilliant dyes have led to their widespread usage in reagent panels. However, cocktailing different reagents is time-consuming and error-prone, and may result in undesired variability.
To address this issue BD has developed an innovative technology that allows cocktailing of up to 5 BD Horizon™ Brilliant reagents. This means that customers can now order cocktails containing up to 14-16 reagents, including up to 5 BD Horizon™ Brilliant reagents, in a stable, dried-down format in a single tube.
To demonstrate the feasibility of this technology, the authors of this poster have designed 2 panels of 5 and 7 colours , for the identification of regulatory T cells (Tregs) and the characterisation of their different Treg subsets. These include IL-17 producing Tregs and CD15s+ Tregs. They have compared the performance of the 7-colour panel in both the dried-down and liquid formats, and have demonstrated that the dried-down format is stable and comparable in performance to the liquid. The percentage of positive cells measured is also comparable between the two forms of the reagent.