Development of a dried-down, multicolour reagent solution for enhanced flow-cytometric applications

DriChroma CD4, flowcytometry

Multicolour flow cytometry is possibly the most powerful tool for identifying, characterising and phenotyping cells. Dyes are a very important component of multicolour flow cytometry. The brightness and high performance of BD Horizon™ Brilliant dyes have led to their widespread usage in reagent panels. However, cocktailing different reagents is time-consuming and error-prone, and may result in undesired variability.

To address this issue BD has developed an innovative technology that allows cocktailing of up to 5 BD Horizon™ Brilliant reagents. This means that customers can now order cocktails containing up to 14-16 reagents, including up to 5 BD Horizon™ Brilliant reagents, in a stable, dried-down format in a single tube.

To demonstrate the feasibility of this technology, the authors of this poster have designed 2 panels of 5 and 7 colours , for the identification of regulatory T cells (Tregs) and the characterisation of their different Treg subsets. These include IL-17 producing Tregs and CD15s+ Tregs. They have compared the performance of the 7-colour panel in both the dried-down and liquid formats, and have demonstrated that the dried-down format is stable and comparable in performance to the liquid. The percentage of positive cells measured is also comparable between the two forms of the reagent.

Learn more in the Development of a Dried-down, Multicolor Reagent Solution for Enhanced Flow-cytometric Applications poster.

 

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Products For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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