Unraveling the whole transcriptome of a single cell is now easier with the BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit. First, the lysis, capture of mRNAs using the bead-based retrieval mechanism of the BD Rhapsody™ and cDNA library preparation are carried out. Following this, the BD™ WTA Amplification Kit is used to generate whole transcriptome libraries using random primers, followed by amplification and addition of sequencing adapters. The BD™ WTA Amplification kit is also compatible with the BD™ AbSeq oligo-conjugated antibodies and with the BD™ Single-Cell Multiplexing Kit.
The whole transcriptome assay allows for the identification of the most differentially expressed genes in the cell. Once identified, these genes can be used to create custom panels with up to 500 targets, or added to up to 100 targets in one of BD’s pre-designated panels to create supplemental panels according to experimental needs.
Learn more in the WTA brochure.