Fluorescence assisted cell-sorting in a oncohematology laboratory

FACS or fluorescence assisted cell sorting, is an efficient technique to sort and select specific cell types from mixed populations. The authors of this poster have carried out molecular biology experiments like fluorescent in-situ hybridization (FISH), TCR re-arrangement and allele specific oligonucleotide tests on bone marrow samples taken from multiple myeloma, B-ALL, B-LS and mastocytosis patients. They have observed that unsorted samples show low infiltrations and enriching cells using cell sorting yields improved results from the assays. All the cell sorting experiments described in the poster were carried out on a BD FACSMelody™ cell sorter. The authors conclude that cell enrichment using FACS is a useful technique in the clinic as it improves the sensitivity of assays, potentially helping in better diagnoses and treatments.

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