Webinar: Considerations when generating diagnostic samples for ccfDNA analysis – Dr. Andrea Ullius, Scientist, Qiagen GmbH

CCFDNA

The testing of ccfDNA or circulating cell-free DNA in liquid biopsies can be useful for the management and analysis of malignant and benign neoplasms. However, the extraction of ccfDNA is challenging due to a variety of factors. The blood sample needs to be stabilised quickly at room temperature to prevent cell apoptosis, an important consideration for reducing background during ccfDNA detection. Also, the ccfDNA in the samples must be prevented from cross-linking.

Haemolysis of red blood cells, that causes the release of intracellular components can also affect ccfDNA analysis outcomes. An effective workflow that includes all the steps from blood drawing to stabilization and analysis is, therefore, an important requirement for effective testing of ccfDNA.

In this webinar, Dr.Andrea Ullius, Scientist, Qiagen GmbH, talks about how ccfDNA in a blood sample can be stabilised for the analysis, and what the best practices for generating high-quality diagnostic samples are.

Watch the webinar 

 

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