Met-Flow: A strategy for single-cell metabolic analysis

Featuring World-Renowned Flow Cytometry Expert:  Dr. John Connolly

Wednesday, October 21, 2020 1 p.m. SGT

Dr. John Connolly

A complex interaction of anabolic and catabolic metabolism

A complex interaction of anabolic and catabolic metabolism underpins the ability of leukocytes to mount an immune response. Their capacity to respond to changing environments by metabolic reprogramming is crucial to effector function. However, current methods lack the ability to interrogate this network of metabolic pathways at a single-cell level within a heterogeneous population. Using Met-Flow, a flow cytometry-based method, the metabolic state of immune cells can be captured by targeting key proteins and rate-limiting enzymes across multiple pathways.

New technologies including antibody panels, flow cytometers and advanced data-analysis tools have been developed to simultaneously measure divergent metabolic profiles and dynamic remodeling in human peripheral blood mononuclear cells. This webinar will provide insight into the complex metabolic state of any cell as it relates to phenotype and function, leading to a greater understanding of the role of metabolic heterogeneity in immune responses.

Key topics covered in this webinar include: 

  • How staining and analysis of metabolic and cell surface proteins can be made simultaneously at a single-cell level
  • How a novel high-parameter flow cytometry method utilizing antibodies against metabolic proteins that are critical and rate-limiting in their representative pathways can shed light into the metabolic state of any given cell type
  • How various advanced data-analysis tools can be utilized to derive unique correlations between immune subpopulations and specific metabolic pathways

Register for the webinar

 

About the speaker

Dr. Connolly is a Research Director for Translational Immunology at the Institute of Molecular and Cellular Biology (IMCB). Additionally, Dr. Connolly serves as the Chief Scientific Officer of Parker Institute for Cancer Immunotherapy, a multidisciplinary center focused on next-generation therapy in immuno-oncology. An Adjunct Associate Professor of Immunology at Baylor University, he served on the Board of Governors for the Institute of Biomedical Sciences. As a human immunologist, his research interests focus on target discovery for immune modulation.

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